Fluorescence spectrometer and infrared spectrometer are common spectral instruments, they are different in the application principle, measurement range, application field and other aspects, the specific differences are as follows:

The principle of use is different:

Fluorescence spectrometers obtain information by measuring the fluorescence emitted by the sample as a result of its absorption of externally excited light, while infrared spectrometers obtain information by measuring the sample’s absorption of infrared light.

The difference between fluorescence spectrometer and infrared spectrometer

Different measuring range:

Fluorescence spectrometers are mainly used to measure the spectrum of fluorescent molecules. They are generally suitable for visible light to ultraviolet light region, that is, wavelength range of 200-800 nanometers. The infrared spectrometer is suitable for the infrared region, generally covering the range of 4000-400 cm ^-1 wave number.

Different application fields:

Fluorescence spectrometer is mainly used in chemistry, biology, materials science and other fields, such as protein structure research, photocatalysis, fluorescence labeling detection, etc. The infrared spectrometer is mainly used in chemical, biological, material, pharmaceutical and other fields, such as compound structure analysis, polymer material detection, drug quality inspection, etc.

Different resolution:

The resolution of fluorescence spectrometer is relatively low, generally about 0.1-1 nm, and the resolution of infrared spectrometer is relatively high, can reach about 0.001 nm.

Sample preparation is different:

The sample of fluorescence spectrometer is usually a solution of liquid or solid sample, which can emit fluorescence signal only under the action of external excitation light. The samples of infrared spectrometer can be liquid, solid or gas samples, usually need to be made into thin films or particles, so as to facilitate the measurement of the absorption and transmission of the samples under infrared light.

Therefore, there are some differences between fluorescence spectrometer and infrared spectrometer in the application principle, measurement range and application field, so it is necessary to select the appropriate instrument according to the actual demand.